Journal: BMC genomics

Article Title: Autophagy is involved in the toxicity of the biocontrol agent GC16 against Tetranychus pueraricola (Acari: Tetranychidae) based on transcriptomic and proteomic analyses.

doi: 10.1186/s12864-025-11312-7

Figure Lengend Snippet: Fig. 6 GC16 induced autophagy in Sf9 cells. (A) Representative fluorescence staining images of the LC3 protein in Sf9 cells treated with GC16 or water (for control, designated as CK) for 24 h (scale bar = 20 μm). The region of the LC3 protein that displays positive staining is observed to be red in color. The staining of the nucleus is visible in blue with DAPI. Autophagic vacuoles were observed to be red in color. (B) Average fluorescence intensity of the LC3 protein in Fig. 6A. Data are represented as mean ± SE of three replicates (*P < 0.05, **P < 0.01, and *** P < 0.001 with compared to control). (C) Western blotting analysis for expression of autophagy marker LC3 in Sf9 cells treated with GC16 for 24 h. β-Actin was used as a loading control. The blots were cropped and in supplementary file the full-length blots/gels are presented. (D) A quantitative analysis of western blot was shown by LC3-II/LC3-I ratios in the down panel. All data are represented as mean ± SE of three experiments in triplicate (*P < 0.05, **P < 0.01, and *** P < 0.001 with compared to control)

Article Snippet: Sf9 cells were cultured on coverslips and treated with water (control) or 2.1 mg/mL GC16 for 24 h. The cells were fixed with 4% paraformaldehyde for 15 min, permeabilized with 0.5% Triton X-100 at 25 °C for 5 min, and then blocked (AR1009; BOSTER Biological Technology Co., Ltd., Wuhan, China) at 25 °C for 30 min. LC3 expression was detected by incubating the samples with primary antibodies against LC3 (AP0762, Bioworld Technology, Inc., USA) at 4 °C overnight and then with corresponding secondary antibodies (BA1032, BOSTER Biological Technology Co. Ltd, Wuhan, China) at 37 °C for 1 h. Subsequently, the samples were stained with DAPI (C1002, Beyotime Biotechnology, Shanghai, China) for 5 min, and covered with a sealing agent to prevent fluorescence quenching (0100- 01, Southern Biotechnology Associates, Inc., USA).

Techniques: Fluorescence, Staining, Control, Western Blot, Expressing, Marker

Journal: Oncotarget

Article Title: Inhibition of primary breast tumor growth and metastasis using a neuropilin-1 transmembrane domain interfering peptide

doi: 10.18632/oncotarget.10101

Figure Lengend Snippet: A. Representative western blots showing the induction of AKT phosphorylation upon treatments with 200ng/ml Sema3A and with or without MTP-NRP1. Akt and p-AKT expression levels were normalized with GAPDH. B. Quantitative analysis of 3 independent experiments showing the relative expression of p-AKT/AKT in the different experimental conditions. (*= p<0.05, ns: not significant; Mann Whitney test).

Article Snippet: MDA-MB-231 cells were seeded on 6-well plates overnight, and then treated with 200ng/ml Sema3A (R&D 1250-S3) for 30 minutes prior to addition of 10 −6 M of MTP-NRP1 for one additional hour.

Techniques: Western Blot, Expressing, MANN-WHITNEY